In isoelectric focusing gel electrophoresis
A) particular care must be taken to ensure the same pH along the length of the gel
B) there is a pH gradient that parallels the electric field gradient
C) the electric current is allowed to fluctuate
D) the electric circuits of the apparatus must be very well insulated

Question

Quizplus · Accepted Answer

In isoelectric focusing gel electrophoresis, a pH gradient is established along the length of the gel, which parallels the electric field gradient. This allows proteins to migrate until they reach a point in the gel where the pH equals their isoelectric point, at which they have no net charge and thus stop moving.

In Isoelectric Focusing Gel Electrophoresis