In an ELISA, the compound 4-chloro-1-naphthol is used because
A) it helps the secondary antibody to bind to the protein
B) it helps the primary antibody bind to the protein
C) it turns color in the presence of an enzyme that is bound to the secondary antibody
D) all of the choices

Question

Quizplus · Accepted Answer

4-chloro-1-naphthol is used as a substrate in ELISA (Enzyme-Linked Immunosorbent Assay) that turns color in the presence of an enzyme (typically horseradish peroxidase or HRP) that is bound to the secondary antibody, allowing for the detection of the antigen-antibody complex.

In an ELISA, the Compound 4-Chloro-1-Naphthol Is Used Because