What effect is seen on a Lineweaver-Burk graph when a non-competitive inhibitor is added?
A) The y-intercept is changed, but not change the slope of the line.
B) The slope of the line is changed, but not the y-intercept.
C) Both the y-intercept and the slope of the line are changed.
D) Neither the y-intercept not the slope of the line is changed.

Question

Quizplus · Accepted Answer

A non-competitive inhibitor binds to the enzyme at a site other than the active site, causing a conformational change that reduces the enzyme's affinity for the substrate. This results in an increase in the Michaelis constant (Km), which is reflected as a change in the y-intercept of the Lineweaver-Burk graph. The slope of the line, which is related to the maximum velocity (Vmax), is not affected by a non-competitive inhibitor.

What Effect Is Seen on a Lineweaver-Burk Graph When a Non-Competitive