The amino acids are initially attached to either the 2' or 3' hydroxyl group of the tRNA.

Name: The amino acids are initially attached to either the 2' or 3' hydroxyl group of the tRNA.
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Amino acids are attached to the 3' hydroxyl group of the adenosine residue at the 3' end of tRNA, but can also form an ester linkage with the 2' hydroxyl group, although the 3' position is the functional site for protein synthesis.

The Amino Acids Are Initially Attached to Either the 2