Exhibit 13A This is a map of the pBR322 plasmid, numbers on the map refer to the base where the site is located. Refer to Exhibit 13A. If a recombinant plasmid was obtained inserting DNA into the Bam HI site, screening for the recombinant plasmid can be done by the following technique.
A) Plate on nutrient agar plates that contain ampicillin.
B) Plate on nutrient agar plates that contain tetracycline.
C) Plate on nutrient agar plates that contain both ampicillin and tetracycline.
D) Plate on nutrient agar plates that contain ampicillin, followed by replica plating on tetracycline.
E) Plate on nutrient agar plates that contain tetracycline, followed by replica plating on ampicillin.

Question

Exhibit 13A This is a map of the pBR322 plasmid, numbers on the map refer to the base where the site is located.  Refer to Exhibit 13A. If a recombinant plasmid was obtained inserting DNA into the Bam HI site, screening for the recombinant plasmid can be done by the following technique.
A) Plate on nutrient agar plates that contain ampicillin. 
B) Plate on nutrient agar plates that contain tetracycline. 
C) Plate on nutrient agar plates that contain both ampicillin and tetracycline. 
D) Plate on nutrient agar plates that contain ampicillin, followed by replica plating on tetracycline. 
E) Plate on nutrient agar plates that contain tetracycline, followed by replica plating on ampicillin.

Quizplus · Accepted Answer

The Answer of Exhibit 13A This is a map of the pBR322 plasmid, numbers on the map refer to the base where the site is located.  Refer to Exhibit 13A. If a recombinant plasmid was obtained inserting DNA into the Bam HI site, screening for the recombinant plasmid can be done by the following technique.
A) Plate on nutrient agar plates that contain ampicillin. 
B) Plate on nutrient agar plates that contain tetracycline. 
C) Plate on nutrient agar plates that contain both ampicillin and tetracycline. 
D) Plate on nutrient agar plates that contain ampicillin, followed by replica plating on tetracycline. 
E) Plate on nutrient agar plates that contain tetracycline, followed by replica plating on ampicillin.

Exhibit 13A This Is a Map of the PBR322 Plasmid