Exhibit 13D The plasmid pSU922 is a circular DNA containing 25000 base pairs. The β -gal gene codes for the enzyme β -galactosidase, the product of which will turn bacterial colonies blue when grown in the presence of X-gal; the Amp^R gene confers ampicillin resistance. Refer to Exhibit 13D. Neglecting any discussion of whether it's a good or bad choice, I attempt to insert a gene fragment into the Hind III site and transform bacteria with the plasmid. How can I tell which transformants have the insert? A) The bacteria will not be able to grow in the presence of ampicillin, and they will be blue. B) The bacteria will not be able to grow in the presence of ampicillin, and they will be white. C) The bacteria will be able to grow in the presence of ampicillin, and they will be blue. D) The bacteria will be able to grow in the presence of ampicillin, and they will be white.

Question

Quizplus · Accepted Answer

The Answer of Exhibit 13D The plasmid pSU922 is a circular DNA containing 25000 base pairs. The β -gal gene codes for the enzyme β -galactosidase, the product of which will turn bacterial colonies blue when grown in the presence of X-gal; the Amp^R gene confers ampicillin resistance. Refer to Exhibit 13D. Neglecting any discussion of whether it's a good or bad choice, I attempt to insert a gene fragment into the Hind III site and transform bacteria with the plasmid. How can I tell which transformants have the insert? A) The bacteria will not be able to grow in the presence of ampicillin, and they will be blue. B) The bacteria will not be able to grow in the presence of ampicillin, and they will be white. C) The bacteria will be able to grow in the presence of ampicillin, and they will be blue. D) The bacteria will be able to grow in the presence of ampicillin, and they will be white.

Exhibit 13D the Plasmid PSU922 Is a Circular DNA Containing