Which of the following conditions is not a requirement to assure production of a protein when a plasmid is being designed to express a eukaryotic protein in a bacterium?
A) Introns must be deleted from the gene.
B) The cloning site must include an RNA polymerase promoter.
C) The mRNA product must contain a ribosome-binding site.
D) Differences in the genetic code between eukaryotes and prokaryotes must be accommodated.
E) All of these conditions must be met.

Question

Quizplus · Accepted Answer

Differences in the genetic code between eukaryotes and prokaryotes are minimal, as the genetic code is nearly universal. The other conditions (A, B, C) are necessary for successful expression of a eukaryotic protein in a bacterium.

Which of the Following Conditions Is Not a Requirement to Assure