Which of the following is required for substrate-level phosphorylation?
A) The substrate must contain multiple phosphate groups.
B) Molecular oxygen must present.
C) The standard free energy of the hydrolysis reaction is more negative than that for hydrolysis of the new phosphate compound being formed.
D) All of the above are necessary for substrate-level phosphorylation.

Question

Quizplus · Accepted Answer

Substrate-level phosphorylation involves the direct transfer of a phosphate group from a substrate to ADP to form ATP. This process does not require molecular oxygen or a substrate with multiple phosphate groups. It is driven by the energy released from a reaction where the standard free energy of the hydrolysis of the substrate is more negative than that of ATP hydrolysis, making the reaction energetically favorable.

Which of the Following Is Required for Substrate-Level Phosphorylation