The NADH used for the reduction reactions during gluconeogenesis usually come from this reaction: A) Gylceraldehyde-3-phosphate dehydrogenase. B) Malate dehydrogenase activity in the cytoplasm. C) Pyruvate carboxylase activity in the mitochondria. D) A variety of reactions which produce NADH in the mitochondria. E) None of these, since it is NAD⁺ that is used in gluconeogenesis.

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Quizplus · Accepted Answer

NADH for gluconeogenesis is primarily generated by the malate dehydrogenase activity in the cytoplasm, which converts malate to oxaloacetate while reducing NAD+ to NADH. This step is crucial for providing the reducing power needed for the gluconeogenic pathway.

The NADH Used for the Reduction Reactions During Gluconeogenesis Usually