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Match the Sequence of the Steps for the Southern Blot

Question 96

Matching

Match the sequence of the steps for the Southern Blot assay.

Premises:

Responses:

Step 3

The blotting buffer transfers DNA fragments to DNA binding filter.

Load the DNA fragments on the gel and separate by electrophoresis.

The radiograph shows the hybridized DNA fragments.

Place the filter and radioactively labeled probe together in the sealed bag.

Visualize the DNA fragments using UV light.

Premises:

Step 3

Responses:

The blotting buffer transfers DNA fragments to DNA binding filter.

Load the DNA fragments on the gel and separate by electrophoresis.

The radiograph shows the hybridized DNA fragments.

Place the filter and radioactively labeled probe together in the sealed bag.

Visualize the DNA fragments using UV light.

Correct Answer:

Premises:

Responses:

The blotting buffer transfers DNA fragments to DNA binding filter.

Load the DNA fragments on the gel and separate by electrophoresis.

The radiograph shows the hybridized DNA fragments.

Place the filter and radioactively labeled probe together in the sealed bag.

Visualize the DNA fragments using UV light.

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