During the preparation of a human genomic library, plasmids containing human DNA fragments are inserted into:
A) compatible human cells
B) an antibiotic-resistant strain of E. coli to protect the inserted fragments
C) antibiotic-sensitive E. coli cells that become antibiotic-resistant if transformed
D) bacteriophages
E) mRNA molecules

Question

Quizplus · Accepted Answer

During the preparation of a human genomic library, plasmids containing human DNA fragments are inserted into antibiotic-sensitive E. coli cells. These cells become antibiotic-resistant if they are successfully transformed with plasmids that carry an antibiotic resistance gene, allowing for the selection of transformed cells.

During the Preparation of a Human Genomic Library, Plasmids Containing