Lederberg and Tatum used the bacterial strains bio- met- leu+ thr+ thi+ and bio+ met+ leu- thr- thi- in their experiments to determine if genetic recombination occurs in bacteria. Would it have been better to use two strains with the genotypes met- leu+ and met+ leu- instead?
A) Yes, because it is easier to study 2 genes than 5 genes.
B) Yes, because the met- leu+ and met+ leu- strains only have one mutation, which makes it easier for genetic recombination to occur.
C) No, because the met- leu+ and met+ leu- strains only have one mutation. A single random mutation could restore methionine or leucine synthesis in the strains.
D) No, because strains with only one nutrient-deficient gene can still grow on minimal medium.
E) No, because cells have two genes that encode different versions of leucine, so the met+ leu- strain would still be able to grow on minimal medium.
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