In Which Situation Would Gene Cloning Occur? ​

In which situation would gene cloning occur? ​

A) a DNA fragment is inserted directly into a bacterium and is replicated
B) a DNA fragment is inserted into a plasmid, which is then introduced into a bacterium where the gene is expressed but the plasmid does not replicate
C) a DNA fragment is inserted into a plasmid, which is then introduced to a bacterium where the plasmid replicates
D) a DNA fragment is inserted directly into a bacterium and is digested by nucleases
E) a protein of interest is taken up by a bacterium, which incorporates the protein into its cell membrane