Identify the correct steps for cloning a gene of interest, then arrange them in the correct order.
1. Transform the bacteria.
2. Incubate the DNA fragments and cut cloning vector with DNA ligase.
3. Use restriction enzymes to cut the gene of interest and the cloning vector.
4. Spread bacteria on medium containing lactose and ampicillin.
5. Spread bacteria on medium containing X-gal and ampicillin.

Question

Identify the correct steps for cloning a gene of interest, then arrange them in the correct order.
1.  Transform the bacteria.
2.  Incubate the DNA fragments and cut cloning vector with DNA ligase.
3.  Use restriction enzymes to cut the gene of interest and the cloning vector.
4.  Spread bacteria on medium containing lactose and ampicillin.
5.  Spread bacteria on medium containing X-gal and ampicillin.

Quizplus · Accepted Answer

The correct order is: 3. Use restriction enzymes to cut the gene of interest and the cloning vector, 2. Incubate the DNA fragments and cut cloning vector with DNA ligase, 1. Transform the bacteria, 5. Spread bacteria on medium containing X-gal and ampicillin.

Identify the Correct Steps for Cloning a Gene of Interest