How did Zhang and Cech purify the RNA molecules that were able to catalyze the linkage between two amino acids?
A) passing the reaction mixture through a column that binds biotin
B) passing the reaction mixture through a column that binds all proteins
C) passing the reaction mixture through a column that binds RNA
D) centrifuging the reaction mixture to concentrate the proteins
E) centrifuging the reaction mixture to concentrate the RNA molecules

Question

Quizplus · Accepted Answer

Zhang and Cech used a biotin-streptavidin system for purification. They attached biotin to the substrate, and after the reaction, molecules that catalyzed the linkage (including the RNA catalysts) could be isolated by passing the mixture through a column that binds biotin, allowing for the separation of active RNA molecules from the rest of the mixture.

How Did Zhang and Cech Purify the RNA Molecules That