In order to elucidate the Calvin cycle, Calvin and his colleagues used two-dimensional paper chromatography to analyze the presence of different molecules under different conditions. What did they conclude from the accumulation of RuBP when CO₂levels were low?

Question

Quizplus · Accepted Answer

RuBP (ribulose bisphosphate) is the first substrate to react with CO₂ in the Calvin cycle, catalyzed by the enzyme rubisco. The accumulation of RuBP when CO₂ levels are low indicates that RuBP is waiting to react with CO₂, confirming its role as the initial substrate in the cycle.

In Order to Elucidate the Calvin Cycle, Calvin and His