Deoxyribonucleotides are included in the Sanger sequencing reaction mix to _____.
A) provide a substrate for RNA polymerase
B) enhance the activity of the dideoxyribonucleotides
C) allow longer DNA fragments (up to 300 additional base pairs) to be sequenced
D) allow the production of multiple DNA fragments of different lengths
E) prevent the DNA polymerase from making too many errors

Question

Quizplus · Accepted Answer

Deoxyribonucleotides serve as the building blocks for DNA polymerase to synthesize new DNA strands during Sanger sequencing. By incorporating these nucleotides, the reaction enables the production of multiple DNA fragments of varying lengths, which are essential for determining the sequence of the target DNA.

Deoxyribonucleotides Are Included in the Sanger Sequencing Reaction Mix to _____