Which element of a dideoxy sequencing reaction allows for visualization of the DNA sequence?
A) the mixture of the four dideoxyribonucleotides, each with a different fluorescent label
B) DNA primer
C) the mixture of the four deoxyribonucleotides
D) DNA polymerase
E) DNA ligase

Question

Quizplus · Accepted Answer

The mixture of the four dideoxyribonucleotides, each with a different fluorescent label, allows for the visualization of the DNA sequence in dideoxy sequencing (also known as Sanger sequencing). These labeled nucleotides terminate DNA synthesis at different points, creating fragments of various lengths that can be visualized and used to determine the DNA sequence.

Which Element of a Dideoxy Sequencing Reaction Allows for Visualization