Which of the following methods of DNA amplification does NOT require cloning?

Question

Quizplus · Accepted Answer

Polymerase chain reaction (PCR) amplifies DNA without the need for cloning by using primers specific to the target sequence to exponentially amplify the DNA. Reverse transcription (RT) can also amplify DNA without cloning, but it first converts RNA into complementary DNA (cDNA) using reverse transcriptase before using PCR to amplify the cDNA. Cloned DNA involves inserting a target DNA sequence into a vector and then amplifying it using bacteria, which is not a method of DNA amplification that does not require cloning. Therefore, the correct answer is B.

Which of the Following Methods of DNA Amplification Does NOT