Identify All Answer Choices That Best Complete the Question

Identify all answer choices that best complete the question. Which of the following techniques are followed when preparing a culture?

A) Any mixed cultures must be separated into subcultures until only one species is present.
B) The sample is spread on the media in a specific pattern that divides the plate into four quadrants.
C) The plate is stored bottom up to prevent condensation from dripping into the colonies.
D) The plate is stored in an incubator to allow optimal growth.