It Can Be Argued That Conflict Between the English Settlers

It can be argued that conflict between the English settlers and local Indians in Virginia became inevitable when:

A) the Native Americans realized that England wanted to establish a permanent and constantly expanding colony,not just a trading post.
B) Pocahontas married John Rolfe.
C) the House of Burgesses passed a law ordering Native Americans out of the colony.
D) Powhatan led an attack against the English settlers in 1644.
E) Spain formed a military alliance with Powhatan.