Researchers lyse human cells and isolate a specific messenger RNA template using gel electrophoresis. Reverse transcription polymerase chain reaction is then used to synthesize complementary DNA (cDN A) from the RNA template. Next, the cDNA is modified into an expression vector containing an optimized bacterial promoter, ribosomal binding site, and terminator sequence. After insertion of the vector into appropriate bacterial hosts, the transformed bacteria are cultured in a bioreactor and produce large quantities of a protein containing a domain that binds to a specific DNA sequence. This protein is most likely the receptor for which of the following hormones?

Question

Researchers lyse human cells and isolate a specific messenger RNA template using gel electrophoresis.  Reverse transcription polymerase chain reaction is then used to synthesize complementary DNA (cDN A) from the RNA template.  Next, the cDNA is modified into an expression vector containing an optimized bacterial promoter, ribosomal binding site, and terminator sequence.  After insertion of the vector into appropriate bacterial hosts, the transformed bacteria are cultured in a bioreactor and produce large quantities of a protein containing a domain that binds to a specific DNA sequence.  This protein is most likely the receptor for which of the following hormones?

Quizplus · Accepted Answer

Eductional Objective: DNA-binding proteins include transcription factors (Myc, CREB), steroid receptors (cortisol, aldosterone, progesterone), thyroid hormone receptor, fat-soluble vitamin receptors (vitamin D, retinoic acid), and DNA transcription and replication proteins.

Researchers Lyse Human Cells and Isolate a Specific Messenger RNA