Put the steps in neuromuscular junction synaptic transmission and EC-coupling in chronological order.
1. Myosin heads hydrolyze ATP during the cross-bridge cycle.
2. An EPSP is generated in the muscle cell.
3. An action potential is conducted along the sarcolemma.
4. Voltage-gated Na+ channels open in sarcolemma.
5. Ca²⁺ rises in the muscle cell cytoplasm.
6. Vesicles containing acetylcholine fuse with axon terminal membrane.
7. Tropomyosin rotates into groove of thin filament.
8. Ca²⁺ dissociates from troponin.
9. Motoneuron axon terminal are depolarized.
10. RyRs open.
11. Acetylcholine receptors open and conduct ions.
12. Ca²⁺+ binds to troponin C.
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