What compounds will come out of an HPLC column first, when the stationary phase is coated with an amino-type compound?
A)Polar
B)Nonpolar
C)High molecular weight
D)Low molecular weight

Question

Quizplus · Accepted Answer

In an HPLC column with an amino-type stationary phase, which is polar, nonpolar compounds will elute first because they have less interaction with the polar stationary phase compared to polar compounds.

What Compounds Will Come Out of an HPLC Column First