How were researchers able to observe active neurons in experiments in learning where doxycycline (DOX) was used with TetTag transgenic mice?

Question

Quizplus · Accepted Answer

B-galactosidase staining is used in conjunction with the TetTag system to identify neurons that were active during a specific learning task. This method allows for the visualization of neurons that have been genetically modified to express a marker (such as LacZ, which encodes the enzyme β-galactosidase) in response to doxycycline administration, enabling researchers to observe and study the patterns of neuronal activation associated with learning and memory.

How Were Researchers Able to Observe Active Neurons in Experiments