Intracellular Staining Is a Major Advance in Cell Sorting That

Intracellular staining is a major advance in cell sorting that

A) requires permeabilization of the cell membrane.
B) allows detection of molecules inside the cell instead of simply at the cell surface.
C) allows subcellular localization of molecules.
D) both requires permeabilization of the cell membrane and allows detection of molecules inside the cell instead of simply at the cell surface.
E) both requires permeabilization of the cell membrane and allows subcellular localization of molecules.