Under Which of the Following Conditions Would T-Cell Activation Be

Under which of the following conditions would T-cell activation be LEAST likely to occur?

A) Macrophages are incubated with antigen, then fixed with paraformaldehyde, then incubated with antigen-specific T cells.
B) Macrophages are fixed with paraformaldehyde, then incubated with antigen, then incubated with antigen-specific T cells.
C) Macrophages are fixed with paraformaldehyde, then incubated with digested antigen, then incubated with antigen-specific T cells.
D) Macrophages are incubated with digested antigen, then fixed with paraformaldehyde, then incubated with antigen-specific T cells.
E) Macrophages are incubated with antigen and then incubated with antigen-specific T cells.