Which of the following statements is TRUE concerning Direct Fluorescent Antibody (DFA) Assay use in microscopy? (Select all that apply)

Question

Quizplus · Accepted Answer

DFA assays involve the use of monoclonal antibodies that are chemically linked to fluorescent dyes (A). When these antibody-dye conjugates bind to their specific target pathogen, they can be visualized under a fluorescence microscope due to the fluorescence emitted by the dye when excited by UV light (B). Fluorescein (which glows green) and rhodamine (which glows red) are indeed among the most commonly used dyes in these assays (C). DFA is not a type of microscope but rather a specific technique used in conjunction with fluorescence microscopy, making statement D incorrect.

Which of the Following Statements Is TRUE Concerning Direct Fluorescent