How Would You Set Up an Experiment (Similar to Those

How would you set up an experiment (similar to those performed by Ehrlich and Goldman) to test whether neural tissue is protected from molecules circulating in the bloodstream? Indicate which step would NOT be necessary.

A) choose a non-polar (lipophilic) dye to inject into animals
B) Inject dyes directly into the CSF
C) Inject dyes directly into the circulation
D) Examine the animal for the presence of dye in neural tissue
E) Examine the animal for presence of dye in non-neural tissues
F) All of the above steps are necessary to set up the experiment