How can one detect alternative splicing of transcripts from a given gene?

Question

Quizplus · Accepted Answer

Alternative splicing leads to the formation of different mRNAs from the same pre-mRNA transcript, therefore comparing the sequences of different mRNAs made from the given gene can detect alternative splicing. Choices A and B are not effective because they do not directly measure alternative splicing. Choice C can detect alternative splicing as well but is less efficient because it requires isolating and analyzing different primary transcripts. Choice E is not relevant to detecting alternative splicing.

How Can One Detect Alternative Splicing of Transcripts from a Given