Which of the following is in the correct order for one cycle of polymerase chain reaction (PCR)?

Question

Quizplus · Accepted Answer

The correct order for one cycle of PCR is denature DNA, anneal primers, and extend primers. This allows for the separation of the double-stranded DNA, annealing of the primers to the single-stranded DNA, and extension of the primers by the Taq polymerase. Adding fresh enzyme and dNTPs are not necessary at each cycle as they are already present in the reaction mixture. Therefore, option D is the correct order for one cycle of PCR.

Which of the Following Is in the Correct Order for One