A fragment cut out of DNA and a plasmid cut open for insertion of the fragment must both have been cut with the same type of restriction enzyme.

Question

Quizplus · Accepted Answer

The restriction enzyme used to cut out the fragment from the DNA and the one used to cut open the plasmid must be the same in order for the fragment to be inserted into the plasmid. This ensures that the sticky ends on the fragment and the plasmid match up and can be ligated together.

A Fragment Cut Out of DNA and a Plasmid Cut