The pH of a solution could affect the ability of EDTA to act as a hexadentate ligand.

Question

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The ability of EDTA to act as a hexadentate ligand depends on the pH of the solution, as the availability of EDTA's lone pairs for complex formation is affected by the concentration of H+ and OH- ions in the solution. At higher pH values, EDTA becomes more negatively charged and can bind more effectively with metal ions, while at lower pH values, it may become protonated and unable to form stable complexes.

The PH of a Solution Could Affect the Ability of EDTA