The pinching off of budding clathrin-coated vesicles is driven by the action of
A) dynamin and GTP hydrolysis.
B) dynamin and ATP hydrolysis.
C) GDP bound ARF.
D) GTP bound ARF.

Question

Quizplus · Accepted Answer

The pinching off of budding clathrin-coated vesicles is driven by the action of dynamin and GTP hydrolysis. This is because dynamin is a GTPase protein that forms a collar around the neck of the budding vesicle, which then undergoes conformational changes and GTP hydrolysis to constrict and finally pinch off the vesicle from the parent membrane. ATP hydrolysis is not involved in this process. The ARF proteins are involved in the formation of COPI and clathrin-coated vesicles. However, the activation of ARF requires GTP binding, not GDP binding. So, neither C nor D are correct answers.

The Pinching Off of Budding Clathrin-Coated Vesicles Is Driven by the Action