A researcher wants to isolate regulatory sequences of DNA bound to their transcription factors. She then wants to sequence the DNA fragments that form the binding sites that the transcription factors recognize and bind. Which technique should the researcher use for her first step?

Question

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Chromatin immunoprecipitation (ChIP) is a technique used to identify DNA sequences bound by specific transcription factors. It involves cross-linking proteins to DNA in cells, followed by sonication to fragment the DNA. Antibodies specific to the transcription factor of interest are then used to immunoprecipitate the protein-DNA complexes, allowing the researcher to isolate the specific DNA sequences bound by the transcription factor. These sequences can then be further analyzed, including by sequencing.

A Researcher Wants to Isolate Regulatory Sequences of DNA Bound