The CRISPR/Cas system has revolutionized the efficiency with which scientists can mutate a gene in vivo to investigate the functional role of the protein it encodes. Which component is not essential in the CRISPR/Cas system?

Question

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The CRISPR/Cas system requires a guide RNA complementary to the target gene sequence (A), the Cas9 nuclease (C) to make cuts in the DNA, and access to the target gene in the DNA (D) to function. It does not act on mRNA (B) but directly on DNA to edit genes.

The CRISPR/Cas System Has Revolutionized the Efficiency with Which Scientists