The three-dimensional structure of a protein is analyzed most definitively by
A) electron microscopy.
B) light microscopy.
C) subcellular fractionation.
D) X-ray crystallography.

Question

Quizplus · Accepted Answer

X-ray crystallography is currently the most definitive method for determining the three-dimensional structure of a protein. This technique involves using X-rays to generate diffraction patterns from a crystal of the protein, which can then be analyzed to determine the atomic coordinates of the protein's constituent atoms. This provides a highly detailed view of the protein's shape and organization, allowing for a better understanding of its function.

The Three-Dimensional Structure of a Protein Is Analyzed Most Definitively