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DNA Is Extracted from a Suspect's Blood by Phenol/chloroform to Determine

Question 41

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DNA is extracted from a suspect's blood by phenol/chloroform to determine if he was involved in a murder case. Thirty µL of the purified DNA is diluted in 970 µL of water (for a final volume of 1000 µL). That dilution is put into a quartz cuvette and its absorbance is read on a spectrophotometer at 260 nm. It gives a reading of 0.25. What is the concentration of DNA in the original sample? (phenol/chloroform extraction results in double stranded DNA use 50 ug DNA / mL = 1 O.D. to calculate concentration)

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"X"ug / mL / 0.25 O.D. = 50 ug...

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