After drug administration has occurred, the amount of drug in the blood that is free to bind at specific target sites is referred to as

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Bioavailability refers to the amount of drug that reaches the systemic circulation and is free to bind at target sites. The therapeutic dose refers to the amount of drug needed to produce a therapeutic effect, first-pass effects refer to the metabolism of a drug before it reaches systemic circulation, and ED₅₀ is not a recognized term in pharmacology.

After Drug Administration Has Occurred, the Amount of Drug in the Blood