Analysis of microbial communities by direct sequencing has numerous advantages over culture-dependent analysis. Which of the following are advantages of culture-based methods over culture-independent analysis? Please select all that apply.

Question

Quizplus · Accepted Answer

Pure cultures can provide useful reference genomes that genomes of less-well-known organisms can be mapped to. However, culture-independent methods usually identify more species than culture-dependent methods and there is no bias towards cultivable organisms. Identification of low-abundance organisms can be difficult with both culture-dependent and culture-independent methods.

Analysis of Microbial Communities by Direct Sequencing Has Numerous Advantages