What technique is most commonly used to sequence entire genomes?
A) the whole-genome shotgun method
B) the nucleic acid probe method
C) the plasmid method
D) the genetic marker method

Question

Quizplus · Accepted Answer

The whole-genome shotgun method is the most commonly used technique to sequence entire genomes. This method involves randomly breaking the genomic DNA into small fragments, sequencing each fragment, and then using computer algorithms to assemble the sequences into a final genome. This method has been used to sequence the genomes of many organisms, including humans, mice, plants, and bacteria.

B, C, and D are not the most commonly used techniques for sequencing entire genomes. The nucleic acid probe method involves using specific probes to target and sequence specific regions of the genome. The plasmid method involves cloning fragments of genomic DNA into plasmids and then sequencing the cloned regions. The genetic marker method involves using specific genetic markers as reference points to sequence adjacent regions of the genome. While these methods can be useful in certain contexts, they are not typically used for sequencing entire genomes.

What Technique Is Most Commonly Used to Sequence Entire Genomes