The application of gel electrophoresis (PAGE) in DNA sequencing
A) is nonexistent because DNA fragments differ widely in charge.
B) depends on separation of similar charge\mass ratio on the basis of size.
C) depends on separation of identical size on the basis of charge\mass ratio.
D) is nonexistent because DNA fragments have no net charge.

Question

Quizplus · Accepted Answer

Gel electrophoresis, including PAGE (polyacrylamide gel electrophoresis), separates DNA fragments based on size, with all fragments having a similar charge-to-mass ratio due to the uniform structure of DNA. This allows for the determination of the length of DNA fragments, which is crucial in DNA sequencing.

The Application of Gel Electrophoresis (PAGE) in DNA Sequencing