Which of the following parameters affects the distance DNA molecules migrate during electrophoresis, at pH = 8?
A) The mass of the DNA
B) The total ionic charge on the DNA molecule
C) The fact that each nucleotide contributes one negative charge at this pH.
D) The concentration of agarose or polyacrylamide in the gel.
E) All of these features control the distance the DNA migrates.

Question

Quizplus · Accepted Answer

All the listed parameters influence the migration distance of DNA molecules during electrophoresis at pH 8. The mass of the DNA affects its mobility, with smaller fragments moving faster. The total ionic charge impacts how the DNA interacts with the electric field. Each nucleotide contributing one negative charge at this pH affects the molecule's overall charge. Finally, the concentration of agarose or polyacrylamide in the gel influences the pore size, affecting the ease with which DNA molecules of different sizes can move through the gel.

Which of the Following Parameters Affects the Distance DNA Molecules