Exhibit 13D?The plasmid pSU922 is a circular DNA containing 25000 base pairs. The β-gal gene codes for the enzyme β-galactosidase, the product of which will turn bacterial colonies blue when grown in the presence of X-gal; the Amp R gene confers ampicillin resistance.

-Refer to Exhibit 13D. Which restriction site is best for inserting a DNA fragment for analysis?
A) BamHI
B) EcoRI
C) HindIII
D) They're all equally good.

Question

Exhibit 13D?The plasmid pSU922 is a circular DNA containing 25000 base pairs. The β-gal gene codes for the enzyme β-galactosidase, the product of which will turn bacterial colonies blue when grown in the presence of X-gal; the Amp R gene confers ampicillin resistance.
 
-Refer to Exhibit 13D. Which restriction site is best for inserting a DNA fragment for analysis?
A) BamHI 
B) EcoRI 
C) HindIII 
D) They're all equally good.

Quizplus · Accepted Answer

HindIII is the best restriction site for inserting a DNA fragment for analysis because it cuts DNA at a specific sequence, which makes it easier to insert the fragment into a plasmid or other vector.

Exhibit 13D?The Plasmid PSU922 Is a Circular DNA Containing 25000