Exhibit 13D?The plasmid pSU922 is a circular DNA containing 25000 base pairs. The β-gal gene codes for the enzyme β-galactosidase, the product of which will turn bacterial colonies blue when grown in the presence of X-gal; the Amp R gene confers ampicillin resistance.

-Refer to Exhibit 13D. Neglecting any discussion of whether it's a good or bad choice, I attempt to insert a gene fragment into the HindIII site and transform bacteria with the plasmid. How can I tell which transformants have the insert?

Question

Exhibit 13D?The plasmid pSU922 is a circular DNA containing 25000 base pairs. The β-gal gene codes for the enzyme β-galactosidase, the product of which will turn bacterial colonies blue when grown in the presence of X-gal; the Amp R gene confers ampicillin resistance.
 
-Refer to Exhibit 13D. Neglecting any discussion of whether it's a good or bad choice, I attempt to insert a gene fragment into the HindIII site and transform bacteria with the plasmid. How can I tell which transformants have the insert?

Quizplus · Accepted Answer

The bacteria with the insert will disrupt the lacZ gene, resulting in white colonies on X-gal plates, and they will grow in the presence of ampicillin due to the plasmid's resistance gene.

Exhibit 13D?The Plasmid PSU922 Is a Circular DNA Containing 25000