In mitosis, chromosomes become aligned at the middle of the cell during

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Metaphase. In metaphase, the spindle is formed and chromosomes become aligned along spindles in the middle of the cell.
Key Takeaway: Mitosis is the process by which a mother cell divides into two identical daughter cells. Mitosis begins with prophase, in which centrioles move to opposite ends of the cell. This is followed by metaphase, where chromosomes become aligned at the center of the cell. Chromosomes move to the oppose ends of the cell in anaphase. During telophase, the chromosomes are enclosed a nuclear membrane. Interphase describes the processes that take place before mitosis such as DNA replication that are essential to the mitotic process.

In Mitosis, Chromosomes Become Aligned at the Middle of the Cell