Figure 15.2

The following questionsrefer to the figure above illustrating the initial steps in the formation of recombinant DNA.
-What is used to cut the DNA in panels 1 and 2?
A) restriction enzymes
B) reverse transcriptase
C) DNA polymerases
D) RNA polymerases
E) DNA ligases

Question

Figure 15.2
​
  
The following questionsrefer to the figure above illustrating the initial steps in the formation of recombinant DNA.
-What is used to cut the DNA in panels 1 and 2?​
A) ​restriction enzymes 
B) ​reverse transcriptase 
C) ​DNA polymerases 
D) ​RNA polymerases 
E) ​DNA ligases

Quizplus · Accepted Answer

Restriction enzymes are used to cut DNA at specific sequences, which is illustrated in panels 1 and 2.

Answer: E 
 DNA ligases are enzymes that can join two pieces of DNA together by forming a phosphodiester bond between the 3' hydroxyl group of one nucleotide and the 5' phosphate group of another nucleotide. This step is shown in panel 3, where the recombinant DNA molecule is formed by joining the cut plasmid and foreign DNA fragments with the help of DNA ligase.

Figure 15.2 ​

Figure 15.2