The most efficient method of determining the DNA sequence of a gene is to
A)insert the ligase gene.
B)expose it to radioactivity.
C)use an automated sequencing machine.
D)hand count the base pairs.

Question

Quizplus · Accepted Answer

Using an automated sequencing machine is the most efficient method for determining the DNA sequence of a gene. This machine can rapidly determine the order of nucleotides in a DNA molecule, with high accuracy and low error rate. Inserting the ligase gene or exposing the DNA to radioactivity are not direct methods of sequencing and can cause DNA damage. Hand counting the base pairs is time-consuming and prone to error.

The Most Efficient Method of Determining the DNA Sequence of a Gene