The PCR process requires three steps. In the first step, the temperature is usually set at 95?C because this is the optimum temperature for the

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The first step in the PCR process involves denaturing the DNA double strands, which is achieved by heating the reaction mixture to 95°C. This high temperature breaks the hydrogen bonds between the two strands of the DNA molecule, resulting in single-stranded DNA templates necessary for the next step of PCR.

The PCR Process Requires Three Steps