Which of the Following Was a Reason for the Decline

Which of the following was a reason for the decline of Italian Renaissance in the sixteenth century?

A) Italy became unified as a large state that was rich in art, trade, and commerce.
B) The Turks invaded Italy, sacking Rome in 1527.
C) In 1494 a Spanish army crossed the Alps, and Italy became a bone of contention between Germany and Spain.
D) Italy became vulnerable to the large political and military institutions from Spain and the north.